prof. dr hab. Andriy Sybirnyy

  • Jednostka:
    Instytut Biotechnologii
  • Budynek: D10
  • Pokój: 117
  • Nr telefonu: 177854945
  • Email: [email protected]
  • ORCID: 0000-0001-8579-1566
  • Konsultacje: poniedziałek, 8.00-9.30

Informacje

I work in the fields of yeast cell biology and biotechnology using different non-conventional yeasts: methylotrophic (Pichia pastoris, Ogataea polymorpha) and flavinogenic (Candida famata). I study mechanisms of autophagy using model organism P. pastoris. Among the most important achievements, the discovery of new genes involved in autophagy should be mentioned< namely ATG26, ATG28 and ATG35, as well as other genes involved in autophagy like TRS85, GSS1. In the field of yeast biotechnology, I study riboflavin synthesis in the yeast C. famata. Earlier, I identified genes SEF1, SFU1, VMA1, MET2 involved in the regulation of riboflavin synthesis; besides, I have constructed efficient overproducers of flavin coenzymes FMN and FAD. I also study alcoholic fermentation major lignocellulosic sugars, mostly xylose, and glucose. Using the approach of metabolic engineering and a random selection, the strains of the thermotolerant yeast O. polymorpha have been constructed which accumulate 50 times more ethanol from xylose relative to the wild-type strain. Among many novel selection approaches, the methods of positive selection of ethanol overproducers have been developed. I also have constructed strains of O. polymorpha producing elevated ethanol concentrations for crude glycerol as well as the first anaerobic producers of glycerol in yeasts. In O. polymorpha, I also work on studying glutathione synthesis and constructed one of the most efficient producers of this tripeptide in yeasts. I am also the author of the overproducer of the recombinant anticancer protein arginine deiminase and hepatitis B virus surface antigen (HBsAg). The results of my study have been published in international research journals like Microbiology and Molecular Biology Reviews (IF 15,2), Trends in Biotechnology (IF 14,5). FEMS Microbiology Reviews (IF 13,9), PNAS (IF 10,0), Developmental Cell (IF 9,8), Autophagy (IF 9,2), Metabolic Engineering (IF 7,6), and others.

I am a member of the European Academy of Microbiology (EAM) – prestige organization established due to FEMS (Federation of European Microbiological Societies) initiative, the aim of EAM is to strengthen the influence of microbiology in Europe.

I am FEMS Director for Microbiology Education and Public Engagements.

I am the member of the Editorial Boards of international scientific journals: Yeast, FEMS Yeast Research, Cell Biology International. I also acted for 10 years (till 2013) as a member of the Editorial Board of Autophagy.

I participated in and/or organized many scientific meetings, including 13, 14 International Congresses on Yeasts (Madison, USA, 2012; Awaji island, Japan, 2016), International Specialized Symposia on Yeasts in 2011 (Guadalajara, Mexico); 2013 (Stara Lesna, Slovakia); 2014 (Vipava, Slovenia); 2015 (Perugia, Italy); in 4th, 5th, 6th Polish-Ukrainian (now, international) R. Weigl conferences in Wroclaw (2011), Chernivtsi (2013), Gdansk (2015) and Lodz (2019) and many other meetings. Presented seminars in many institutions, including that in Brussels, Belgium (2011), Vienna, Austria (2012), Gembloux, Belgium (2015, Gatersleben, Germany (2015), Haifa, Israel (2012, 2013, 2015, 2016) and others. Was the organizer of the research meetings: Non-conventional Yeast – from Basic research to application – May 15-18, 2019 in Rzeszow and the 1st Polish Yeast Conference, June 22-24, 2022, in Rzeszow.

Principal Investigator of NCN projects in the frames of the OPUS program:

  1. Title: New enzymatic and biosensor methods for formaldehyde and methanol monitoring in the environment and cellular systems for formaldehyde detoxification (KBN 3PO4B00323)/ Assigned sum:  245 000 PLN, Project start:  2002-10-23 , Project ends:  2006-06-30
  2. Title: Application of the yeast metabolic engineering for construction of the advanced ethanol producers (2012/07/N/NZ9/02137)/ Assigned sum:  350 000 PLN, Project start: 2013-02-24, Project ends: 2016-02-25
  3. Title: The role of transcription factors in the regulation of glucose and xylose metabolism and fermentation in the non-conventional yeast Ogataea polymorpha (2016/21/B/NZ1/00280)/ Assigned sum:  841 400 PLN, Project start: 2017-03-09, Projects ends: 2020-03-08
  4. Title: Regulatory mechanisms involved in riboflavin overproduction in the flavinogenic yeast Candida famata (2018/29/B/NZ1/01497)/ Assigned sum:  975 600 PLN, Project start: 2019-02-13, Project ends: 2022-02-12
  5. Title: Genetic control of metabolic and alcoholic fermentation of pentoses (D-xylose, L-arabinose) in the thermotolerant yeast Ogataea polymorpha (2020/37/B/NZ1/02232)/ Assigned sum:  1 669 200PLN, Project start: 2021-02-15, Project ends: 2024-02-14
  6. Title: Construction and properties of the recombinant yeast strains producing bacterial antibiotics roseoflavin and aminoriboflavin (2021/41/B/NZ1/01224)/ Assigned sum: 1 365 100 PLN, Project start: 2022-01-11, Project ends: 2025-01-10

I am the author of numerous patents, including 6 US, Japanese, and South Korean patents.

I was the Principal Investigator of the proposal by Joint Genome Institute (USA) on sequencing and annotation of the genome of the yeast Ogataea polymorpha. Due to the success of this proposal, O. polymorpha genome is publicly available.

Publikacje

Linki