The guest for the next series of talks on research in the discipline of biotechnology is Latifa Hajri doctoral student in biological studies at the Faculty of Sciences of Bizerte - University of Carthage (Tunisia), who works in the research unit of integrated physiology under the supervision of Prof. Mohammed Ammari. Her Ph.D. thesis focuses on studying the therapeutic effect of a plant called Pearl Millet (which is a very common plant in Tunisia). Latifa conducts research at the Institute of Biotechnology in the Wnuk research group (from September to the end of December 2023)

Please tell us why you have chosen the Institute of Biotechnology UR and the Wnuk research group?

I was actively seeking a laboratory to conduct an experience in cell culture. During my search, I came across several papers related to this field, and I found the research papers of Professor Maciej Wnuk particularly intriguing. Impressed by the work and expertise demonstrated in the papers, I decided to reach out and express my interest in joining in for an internship. After sending an email, I am pleased to say that Professor Wnuk kindly accepted my request

What do you do during your stay in our labs and do you learn a lot of new skills? Has the University of Rzeszow met your expectations?

I'm doing well in the labs, and I try to do my best to learn fast, and I've been lucky to learn a variety of new skills during my time here. Also, everyone here has been incredibly friendly and supportive, making my experience enjoyable The environment at the University of Rzeszow has exceeded my expectations. I appreciate the opportunities provided and am grateful for the chance to be part of such a vibrant academic community.

What laboratory techniques have you already had the opportunity to work with?

I have had the opportunity to work on cell culture using different cell lines, RNA extraction, and I have gained some exposure to advanced techniques such as FISH (Fluorescence In Situ Hybridization), as well as RT-PCR and Western Blot.

What does your work consists of and what you are currently doing in the lab?

My current work in the lab primarily involves preparing an extract from my plant. Once the extract is ready, I test different concentrations of it on various cell lines. The objective is to assess the impact of the extract on these cell lines, particularly in terms of its potential anti-cancer activity. Following the treatment, we employ a range of techniques to analyze the efficiency of the extract and its potential anti-cancer properties.

Why Pearl Millet is such an interesting plant for you that you started to study it?

 I am particularly drawn to the study of the therapeutic effects of Pearl Millet due to its widespread consumption in Tunisia and Africa, especially among economically disadvantaged communities. While it serves as a staple cereal, there is limited research, particularly in areas such as in vitro studies and the exploration of bioactive compounds. And to contribute to understanding the potential medical benefits of Pearl Millet, especially its anti-inflammatory properties and nutritional applications.